How I can remove the latex of Euphorbia tirucalli for phytochemical screening?
I wanted to extract from this plant for the detection of antimicrobial activity due to its traditional demand in certain regions of the world as a bandage.
You will find the publications to remove vegetable material water, ethanol 90% and some organic solvents. Doing it with water, cut and boil slowly for about 0.5 to 1 hour, filter and let it dry stirring, most sprinkled with water. With ethanol is from May to July mazerated day filtered and concentrated by distillation. But I am sure that the substances in these extracts are different. If you want the pure latex, cut pieces of the plant and Waite until the sap is down. In this way, some will stay in the factory, but no substances extraction in plant tissues. But beware, the latex is very irritating to the skin and can cause temporary blindness. Here you can find http://www.fao.org/docrep/X5327e/x5327e12.htm many links to the content included Euphorbiaceae. Aveloz: http://www.euphorbia.de/res_liste.htm
Staphylococcus aureus – Zonex
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